Anti-Ndufs4 抗体 [2C7CD4AG3] (ab87399)
Key features and details
- Mouse monoclonal [2C7CD4AG3] to Ndufs4
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG1
製品の概要
-
製品名
Anti-Ndufs4 antibody [2C7CD4AG3]
Ndufs4 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2C7CD4AG3] to Ndufs4 -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt, WBmore details -
種交差性
交差種: Mouse, Rat, Cow, Human -
免疫原
Recombinant full length protein. This information is considered to be commercially sensitive.
-
ポジティブ・コントロール
- WB: isolated mitochondria from human, bovine, rat, and mouse heart tissue. Flow Cyt: HepG2 cells.
-
特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES, Sodium chloride -
Concentration information loading...
-
精製度
IgG fraction -
特記事項(精製)
Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
ポリ/モノ
モノクローナル -
クローン名
2C7CD4AG3 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab87399の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt | (1) |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB | (4) |
Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
|
特記事項 |
---|
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa). |
ターゲット情報
-
機能
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. -
関連疾患
Defects in NDUFS4 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. -
配列類似性
Belongs to the complex I NDUFS4 subunit family. -
細胞内局在
Mitochondrion inner membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 327680 Cow
- Entrez Gene: 4724 Human
- Entrez Gene: 17993 Mouse
- Entrez Gene: 499529 Rat
- Omim: 602694 Human
- SwissProt: Q02375 Cow
- SwissProt: O43181 Human
- SwissProt: Q9CXZ1 Mouse
see all -
別名
- AQDQ antibody
- CI 18 antibody
- CI 18 kDa antibody
see all
画像
-
All lanes : Anti-Ndufs4 antibody [2C7CD4AG3] (ab87399)
Lane 1 : Isolated mitochondria from human heart at 15 µg
Lane 2 : Isolated mitochondria from bovine heart at 6 µg
Lane 3 : Isolated mitochondria from rat heart at 30 µg
Lane 4 : Isolated mitochondria from mouse heart at 30 µg
Predicted band size: 20 kDa
Observed band size: 20 kDa -
Overlay histogram showing HepG2 cells stained with ab87399 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab87399, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (18)
ab87399 は 18 報の論文で使用されています。
- Liu Y et al. Gene therapy restores mitochondrial function and protects retinal ganglion cells in optic neuropathy induced by a mito-targeted mutant ND1 gene. Gene Ther 29:368-378 (2022). PubMed: 35383288
- Pagano Zottola AC et al. Expression of Functional Cannabinoid Type-1 (CB1) Receptor in Mitochondria of White Adipocytes. Cells 11:N/A (2022). PubMed: 36010658
- Bertan F et al. Comparative analysis of CI- and CIV-containing respiratory supercomplexes at single-cell resolution. Cell Rep Methods 1:100002 (2021). PubMed: 35474694
- Esparza-Moltó PB et al. Generation of mitochondrial reactive oxygen species is controlled by ATPase inhibitory factor 1 and regulates cognition. PLoS Biol 19:e3001252 (2021). PubMed: 33983919
- Hernansanz-Agustín P et al. Na+ controls hypoxic signalling by the mitochondrial respiratory chain. Nature 586:287-291 (2020). PubMed: 32728214