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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly
Overview
Product name
Nck protein (His tag)
Nck 抗体 (3件) 一覧
Protein description
Recombinant Nck fusion protein. Fusion: His-tag at N-terminus.
Expression host
E. coli
Properties
Purity
>95% by SDS-PAGE
Form
Liquid
Storage instructions
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.002% Sodium Azide
Constituents: 3mM Sodium chloride, 2.5mM Imidazole, 10mM Tris, pH 8.0
Concentration
Concentration information loading...
Research areas
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Other
Cancer >> Signal transduction >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Adapters >> Cytoplasmic
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly
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SDS-PAGE - Nck protein (ab40622)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab40622 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SDS-PAGE: Use at an assay dependent dilution.
MS: Use at an assay dependent dilution.
Protein info
Function
Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2.
Sequence similarities
Contains 1 SH2 domain.
Contains 3 SH3 domains.
Post-translational
modifications
Phosphorylated on Ser and Tyr residues.
Cellular localization
Cytoplasm. Endoplasmic reticulum. Nucleus. Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments.
Target information above from: UniProt accessionP16333
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Nck protein (His tag) images:
SDS-PAGE - Nck protein (ab40622)
Analysis of Nck Recombinant Protein (1µg). 4-20% SDS gradient gel. Coomassie blue staining.
References for Nck protein (His tag) (ab40622)
ab40622 has not yet been referenced specifically in any publications.
Publishing research using ab40622? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"