Nck overexpression 293T lysate (whole cell) (ab94184)
製品の概要
-
製品名
Nck overexpression 293T lysate (whole cell)
Nck ライゼート 製品一覧 -
特記事項
ab94184 is a 293T cell transfected lysate in which Human Nck has been transiently over-expressed using a pCMV-Nck plasmid. The lysate is provided in 1X Sample Buffer.
-
アプリケーション
適用あり: WBmore details
製品の特性
-
Mycoplasma free
Yes -
製品の状態
Liquid -
保存方法
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
バッファー
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
研究分野
-
背景
Function: Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Similarity: Contains 1 SH2 domain. Contains 3 SH3 domains. PTM: Phosphorylated on Ser and Tyr residues.
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab94184の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use at an assay dependent dilution.
|
特記事項 |
---|
WB
Use at an assay dependent dilution. |
画像
-
ab94184 at 15 μg/lane on an SDS-PAGE gel
-
All lanes : Anti-Nck antibody (ab55499) at 1/500 dilution
Lane 1 :Nck overexpression 293T lysate (whole cell) (ab94184)
Lane 2 : 293T Non Transfected Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab94184 は論文での使用が確認できていません。