Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (NSC derived neurons)
Permeabilization
Yes - Triton X-100
Specification
NSC derived neurons
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% FCS 0,1% Triton in PBS
Dilution
1/200
Secondary antibody
Dilution
1/100
Name
Non-Abcam antibody was used: AffiniPure Donkey Anti-Mouse IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Thomas Klein
Verified customer
投稿 Nov 13 2015