Anti-Nav1.7 抗体 [N68/6] (ab85015)
Key features and details
- Mouse monoclonal [N68/6] to Nav1.7
- Suitable for: IHC-P
- Reacts with: Mouse
- Isotype: IgG1
製品の概要
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製品名
Anti-Nav1.7 antibody [N68/6]
Nav1.7 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [N68/6] to Nav1.7 -
由来種
Mouse -
特異性
Some customers have used this antibody successfully in rat, however our latest tests were unsuccessful and therefore we can no longer guarantee the reactivity of this species.
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アプリケーション
適用あり: IHC-Pmore details
適用なし: Flow Cyt -
種交差性
交差種: Mouse
交差が予測される動物種: Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- IHC-P: Mouse dorsal root ganglion tissue; Mouse back skin tissue.
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特記事項
The clone number has been updated from S68-6 to N68/6, both clone numbers name the same antibody clone.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
N68/6 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85015の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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IHC-P
Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient (PubMed:7720699, PubMed:17167479, PubMed:25240195, PubMed:26680203, PubMed:15385606, PubMed:16988069, PubMed:17145499, PubMed:19369487, PubMed:24311784). It is a tetrodotoxin-sensitive Na(+) channel isoform (PubMed:7720699). Plays a role in pain mechanisms, especially in the development of inflammatory pain (PubMed:17167479, PubMed:17145499, PubMed:19369487, PubMed:24311784). -
組織特異性
Expressed strongly in dorsal root ganglion, with only minor levels elsewhere in the body, smooth muscle cells, MTC cell line and C-cell carcinoma. Isoform 1 is expressed preferentially in the central and peripheral nervous system. Isoform 2 is expressed preferentially in the dorsal root ganglion. -
関連疾患
Primary erythermalgia
Indifference to pain, congenital, autosomal recessive
Paroxysmal extreme pain disorder
Generalized epilepsy with febrile seizures plus 7
Febrile seizures, familial, 3B -
配列類似性
Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.7/SCN9A subfamily.
Contains 1 IQ domain. -
ドメイン
The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position. -
翻訳後修飾
Phosphorylation at Ser-1490 by PKC in a highly conserved cytoplasmic loop increases peak sodium currents.
Ubiquitinated by NEDD4L; which may promote its endocytosis. Does not seem to be ubiquitinated by NEDD4. -
細胞内局在
Cell membrane. Cell projection. In neurite terminals. - Information by UniProt
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参照データベース
- Entrez Gene: 6335 Human
- Entrez Gene: 20274 Mouse
- Entrez Gene: 78956 Rat
- Omim: 603415 Human
- SwissProt: Q15858 Human
- SwissProt: Q62205 Mouse
- SwissProt: O08562 Rat
- Unigene: 439145 Human
see all -
別名
- ETHA antibody
- GEFSP7 antibody
- hNE Na antibody
see all
画像
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IHC image of Nav1.7 staining in mouse dorsal root ganglion formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85015, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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ab85015 at 1/100 dilution staining Nav1.7 in mouse back skin tissue section by IHC-P. Bouin's fixed and paraffin-embedded tissue sections were used. Tissue underwent heat mediated antigen retrieval in microwave with two, 5 minutes incubation intervals in citrate buffer. A Fluorophore conjugated goat anti mouse at 1/50 dilution was used as secondary.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab85015 は 9 報の論文で使用されています。
- Gonzales J et al. Bimodal Imaging of Mouse Peripheral Nerves with Chlorin Tracers. Mol Pharm 18:940-951 (2021). PubMed: 33404254
- Tian JJ et al. Upregulation of Nav1.7 by endogenous hydrogen sulfide contributes to maintenance of neuropathic pain. Int J Mol Med 46:782-794 (2020). PubMed: 32468069
- Jia Q et al. Activating Sirt1 by resveratrol suppresses Nav1.7 expression in DRG through miR-182 and alleviates neuropathic pain in rats. Channels (Austin) 14:69-78 (2020). PubMed: 32089065
- Gonzales J et al. Fluorescence labeling of a NaV1.7-targeted peptide for near-infrared nerve visualization. EJNMMI Res 10:49 (2020). PubMed: 32409881
- Gonzales J et al. Fluorescence Imaging of Peripheral Nerves by a Nav1.7-Targeted Inhibitor Cystine Knot Peptide. Bioconjug Chem 30:2879-2888 (2019). PubMed: 31647222
- Xie MX et al. Nuclear Factor-kappaB Gates Nav1.7 Channels in DRG Neurons via Protein-Protein Interaction. iScience 19:623-633 (2019). PubMed: 31446225
- Hofmann L et al. Characterization of small fiber pathology in a mouse model of Fabry disease. Elife 7:N/A (2018). PubMed: 30328411
- Bi RY et al. Estradiol upregulates voltage-gated sodium channel 1.7 in trigeminal ganglion contributing to hyperalgesia of inflamed TMJ. PLoS One 12:e0178589 (2017). IHC-Fr ; Rat . PubMed: 28582470
- Bi RY et al. Involvement of trigeminal ganglionic Nav 1.7 in hyperalgesia of inflamed temporomandibular joint is dependent on ERK1/2 phosphorylation of glial cells in rats. Eur J Pain 17:983-94 (2013). PubMed: 23242737