Application
Western blot
Sample
Mouse Cell lysate - whole cell (Intestinal Myofibroblasts)
Loading amount
30 µg
Specification
Intestinal Myofibroblasts
Gel Running Conditions
Reduced Denaturing (7.5 % gel)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/500
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 25°C · Diluent: PBS-T
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL+
Exposure
15 second(s)
Bands
Specific: 60 kDa Non-specific: 30, 40, 80 kDa
Positive control
Brain Tissue
Negative control
N/A
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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投稿 Dec 18 2012