Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (lung)
Specification
lung
Fixative
Formaldehyde
Permeabilization
Yes - with Tris and Tween
Blocking step
Peroxidase as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Other product details
Dilution
1/200
Incubation time
30 minute(s) · Temperature: 24°C · Diluent: in diluent from Dako
Secondary antibody
Name
Non-Abcam antibody was used: labelled polymer, (Histofine)
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Abcam response
Thank you for your review. We have not previously tested Frozen sections althoughtypically we would expect staining in frozen sections in the case where the antibody has been validated for paraffin embedded sections. I would recommend an overnight incubation with the primary antibody to improve your staining.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 May 15 2013