Anti-Nab2 抗体 (ab112056)
Key features and details
- Rabbit polyclonal to Nab2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Nab2 antibody -
製品の詳細
Rabbit polyclonal to Nab2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide, corresponding to a region within amino acids 475-525 of Human Nab2 (NP_005958.1)
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ポジティブ・コントロール
- Whole cell lysate from 293T and Jurkat cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab112056の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 57 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 57 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Isoform 2 lacks repression ability. -
組織特異性
Widely expressed at low levels. Highly expressed in melanoma cell lines. -
配列類似性
Belongs to the NAB family. -
ドメイン
The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization.
The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression. -
細胞内局在
Nucleus. Isoform 2 is not localized to the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 4665 Human
- Entrez Gene: 17937 Mouse
- Entrez Gene: 314910 Rat
- Entrez Gene: 714209 Rhesus monkey
- Omim: 602381 Human
- SwissProt: Q15742 Human
- SwissProt: Q61127 Mouse
- Unigene: 159223 Human
see all -
別名
- EGR 1 binding protein 2 antibody
- EGR-1-binding protein 2 antibody
- EGR1 binding protein 2 antibody
see all
画像
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All lanes : Anti-Nab2 antibody (ab112056) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg/ml
Lane 2 : HeLa whole cell lysate at 15 µg/ml
Lane 3 : HeLa whole cell lysate at 5 µg/ml
Lane 4 : Jurkat whole cell lysate at 50 µg/ml
Predicted band size: 57 kDa
Exposure time: 3 minutes -
Immunoprecipitation of NAB2. Samples: 1 mg of whole cell lysate from 293T cells. NAB2 was detected with anti-NAB2 antibody NAB2 antibody (ab112056) at 1 µg/ml. Lane 1- NAB2 was also immunoprecipitated by rabbit anti-NAB2 antibody NAB2 antibody (ab112056) at 6 µg/mg lysate. Lane 2- NAB2 was immunoprecipitated by an rabbit anti-NAB2 antibody that recognizes an upstream epitope. Lane 3- Rabbit IgG control. Detection: Chemiluminescence with exposure time of 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab112056 は論文での使用が確認できていません。