Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Huh-7.5 (human hepatoma cells))
Permeabilization
No
Specification
Huh-7.5 (human hepatoma cells)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
PFA + glutaraldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Jackson ImmunoResearch AffiniPure
Host species: Goat
Clonality: Polyclonal
Conjugation: Gold 6nm
Host species: Goat
Clonality: Polyclonal
Conjugation: Gold 6nm
Additional data
Additional Notes
This sample has been prepared according to the Tokuyasu method for immune-electro microscopy of cryosections.
PubMed ID
26884193: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Mar 15 2016