Application
Western blot
Sample
Mouse Cell lysate - other (RAW264.7)
Loading amount
40 µg
Specification
RAW264.7
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Concentration
2 µg/ml
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS +0.1% Tween +5% Milk
Secondary antibody
Name
Non-Abcam antibody was used: Anti-mouse IgG, HRP Congugate
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 25-27 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Oct 24 2007