Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart, liver, muscle)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
50 µg
Specification
Heart, liver, muscle
Blocking step
Licor Odyssey buffer with Tween as blocking agent for 30 minute(s) · Concentration: 0.001% · Temperature: 25°C
Other product details
Incubation time
10 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Odyssey block + 1:1000 Tween 20
Dilution
1/1000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Goat anti rabbit 680
Host species: Goat
Clonality: Monoclonal
Conjugation: IRDye 680RD
Host species: Goat
Clonality: Monoclonal
Conjugation: IRDye 680RD
Detection
Detection method
Licor
Negative control
none
Exposure
10 second(s)
Bands
Specific: 119, 80 kDa
Positive control
Human HeLa cell line
Additional data
Additional Notes
1- Heart 50ug
2 Heart 100ug
3 Liver 50ug
4 Liver 100ug
5 muscle 50ug
6 muscle 100ug
7 Human Hela 50ug
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Gillian Hunter
Verified customer
投稿 Nov 28 2017