Anti-NSFL1C 抗体 (ab95963)
Key features and details
- Rabbit polyclonal to NSFL1C
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-NSFL1C antibody -
製品の詳細
Rabbit polyclonal to NSFL1C -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow -
免疫原
Recombinant protein fragment with a sequence corresponding to a region within amino acids 21 and 272 of Human NSFL1C (NP_057227).
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ポジティブ・コントロール
- A431 whole cell lysate (ab7909) can be used as a positive control in WB.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab95963の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 41 kDa.
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IHC-P |
1/100 - 1/250.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 41 kDa. |
IHC-P
1/100 - 1/250. |
ターゲット情報
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機能
Reduces the ATPase activity of VCP. Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER) (By similarity). Inhibits the activity of CTSL (in vitro). -
配列類似性
Belongs to the NSFL1C family.
Contains 1 SEP domain.
Contains 1 UBX domain. -
翻訳後修飾
Phosphorylated during mitosis. Phosphorylation inhibits interaction with Golgi membranes and is required for the fragmentation of the Golgi stacks during mitosis. -
細胞内局在
Nucleus. Golgi apparatus > Golgi stack. Chromosome. Predominantly nuclear in interphase cells. Bound to the axial elements of sex chromosomes in pachytene spermatocytes. A small proportion of the protein is cytoplasmic, associated with Golgi stacks. - Information by UniProt
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参照データベース
- Entrez Gene: 526612 Cow
- Entrez Gene: 55968 Human
- Entrez Gene: 386649 Mouse
- Entrez Gene: 83809 Rat
- Omim: 606610 Human
- SwissProt: Q9UNZ2 Human
- SwissProt: Q9CZ44 Mouse
- SwissProt: O35987 Rat
see all -
別名
- DJ776F14.1 antibody
- MGC3347 antibody
- NSF1C_HUMAN antibody
see all
画像
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All lanes : Anti-NSFL1C antibody (ab95963) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 41 kDa -
ab95963, at 1/100, staining paraffin-embedded differential N/T pair Lung SCC cancer by Immunohistochemistry.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab95963 は 3 報の論文で使用されています。
- Her NG et al. p97 Composition Changes Caused by Allosteric Inhibition Are Suppressed by an On-Target Mechanism that Increases the Enzyme's ATPase Activity. Cell Chem Biol 23:517-28 (2016). PubMed: 27105284
- Davis EJ et al. DVC1 (C1orf124) recruits the p97 protein segregase to sites of DNA damage. Nat Struct Mol Biol 19:1093-100 (2012). WB ; Human . PubMed: 23042607
- Mosbech A et al. DVC1 (C1orf124) is a DNA damage-targeting p97 adaptor that promotes ubiquitin-dependent responses to replication blocks. Nat Struct Mol Biol 19:1084-92 (2012). WB ; Human . PubMed: 23042605