Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Cryopreserved embryonic cortical neurons (QBMcells)
Permeabilization
No
Specification
Cryopreserved embryonic cortical neurons (QBMcells
Fixative
paraformaldehyde with picric acid
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10mM PBS with 0.3% Triton-X
Dilution
1/100
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Donkey anti-rabbit AlexaFluor 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
QBM Cell Science cryopreserved rat cortical neurons cultured for 7 days on poly-D-lysine coated 96 well plates. Very high exposure used to visualize unspecific staining.
Abcam response
Thank you for your valuable feedback. your data suggests that ab79757 may not be suitable to detect NSE in rat embryonic cortical neurons under these experimental conditions. We hope this information is useful to our customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Babben Tinner
Verified customer
投稿 Nov 06 2015