Application
Immunohistochemistry (Frozen sections)
Sample
Zebrafish Tissue sections (retina, neurons)
Permeabilization
Yes - triton X
Specification
retina, neurons
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA blocking Solution
Dilution
1/100
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: invitrogen Alexafluor conjugated goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
No specific staining at 3 and 4dpf. Tris-HCl and Sodium Citrate antigen retrieval were tested.
Abcam response
Thank you very much for your valuable feedback. We have not validated ab79757 for Immunohistochemistry (Frozen sections) in Zebrafish and your data suggests that under these experimental conditions ab79757 may not be suitable to detect NSE in Zebrafish tissue sections. We welcome further customer feedback. We hope this information is useful to our customers. For more information, please contact scientific support.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Sep 05 2014