Anti-NRG3 抗体 [EPR4148] (ab109256)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4148] to NRG3
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-NRG3 antibody [EPR4148]
NRG3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4148] to NRG3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, Flow Cyt (Intra)more details
適用なし: ICC/IF,IHC-P or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Fetal brain, SH-SY5Y, C6, mouse brain and rat brain lysates. Flow Cyt (intra): Permeabilized C6 cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR4148 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109256の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 77 kDa).
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 77 kDa). |
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Direct ligand for the ERBB4 tyrosine kinase receptor. Binding results in ligand-stimulated tyrosine phosphorylation and activation of the receptor. Does not bind to the EGF receptor, ERBB2 or ERBB3 receptors. May be a survival factor for oligodendrocytes. -
組織特異性
Highly expressed in most regions of the brain with the exception of corpus callosum. Expressed at lower level in testis. Not detected in heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, ovary, small intestine, colon and peripheral blood leukocytes. -
配列類似性
Belongs to the neuregulin family.
Contains 1 EGF-like domain. -
発生段階
Isoform 3 is expressed in fetal brain but not in other fetal tissues. -
ドメイン
The cytoplasmic domain may be involved in the regulation of trafficking and proteolytic processing. Regulation of the proteolytic processing involves initial intracellular domain dimerization.
ERBB receptor binding is elicited entirely by the EGF-like domain. -
翻訳後修飾
Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form.
Extensive glycosylation precedes the proteolytic cleavage (By similarity). Isoform 3 is glycosylated. -
細胞内局在
Secreted; Cell membrane. Does not seem to be active and Cell membrane. Isoform 3 is also proteolytically released as a soluble form. - Information by UniProt
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参照データベース
- Entrez Gene: 10718 Human
- Entrez Gene: 18183 Mouse
- Entrez Gene: 498596 Rat
- Omim: 605533 Human
- SwissProt: P56975 Human
- SwissProt: O35181 Mouse
- Unigene: 125119 Human
- Unigene: 258705 Mouse
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別名
- HRG3 antibody
- Neuregulin 3 antibody
- Neuregulin 3 like polypeptide antibody
see all
画像
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All lanes : Anti-NRG3 antibody [EPR4148] (ab109256) at 1/1000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : SH-SY5Y lysate
Lane 3 : C6 lysate
Lane 4 : Mouse brain lysate
Lane 5 : Rat brain lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 77 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted? -
Intracellular flow cytometric analysis of permeabilized C6 (Rat glial tumor cell line) cells using ab109256 (red) or a rabbit IgG (negative) (green).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab109256 は 2 報の論文で使用されています。
- Wang F et al. Resveratrol ameliorates depressive disorder through the NETRIN1-mediated extracellular signal-regulated kinase/cAMP signal transduction pathway. Mol Med Rep 17:4611-4618 (2018). PubMed: 29328454
- Liu Y et al. KRASG12 mutant induces the release of the WSTF/NRG3 complex, and contributes to an oncogenic paracrine signaling pathway. Oncotarget 7:53153-53164 (2016). PubMed: 27449290