Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (adult brain cerebellum)
Specification
adult brain cerebellum
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Other product details
Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Diluent: TBS/BSA/Azide
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/300
Additional data
Additional Notes
I am not familiar with it's distribution in normal adult brain, hence my 3 rating. Others need to confirm/refute the positivity I obtained. It is also expressed in a variety of cells in other brain areas. Please see here for more pictures: http://www.immunoportal.com/index.php look in the IHC image gallery
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
投稿 Nov 09 2007