Application
Western blot
Sample
Monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking Buffer
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Ig
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
Hek293
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: none kDa Non-specific: ~170 kDa
Additional data
Additional Notes
It does not worked for monkey cells.
Abcam response
We would like to thank the reviewer for submitting an Abreview for this product.
As the product had not yet been tested in WB applications with monkey sample types, we were unsure what the result might be; we appreciate the time and effort that the reviewer has put in to testing this product in this application.
Further optimization may be necessary for use of this product in WB applications with monkey samples, and we are happy to help troubleshoot any products or applications which may not be performing.
We do encourage all of our customers to contact us should they wish to test any of our products in untested species or applications, as some our products may qualify for our AbTrial Testing Program.
Should a product fail in a tested application or species, we are always happy to resolve the issue in accordance with our Abpromise Guarantee.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 May 12 2016