Application
Western blot
Sample
Mouse Cell lysate - whole cell (C2C12)
Loading amount
15 µg
Specification
C2C12
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/1000
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: mouse anti-rabbit hrp antibody
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 67 kDa Non-specific: 37, 47, 150 kDa
Additional data
Additional Notes
Too much bands. AND the most obviously band isnt NRF1.
Abcam response
Thank you very much for your feedback.
We are sorry to hear that this antibody did not perform as stated on the datasheet. We are suspecting that this might be a vial- or lot-specific issue.
In order to reduce the non-specific staining possible trouble shooting steps could include : 1) shorten the antibody incubation time, 2) reducing primary and secondary antibody concentrations, and 3) increasing blocking time or try BSA instead of milk as blocking reagent.
Since the customer had contacted us within 120 days of the purchase, a free of charge replacement, credit or refund was offered.
We are sorry to hear that this antibody did not perform as stated on the datasheet. We are suspecting that this might be a vial- or lot-specific issue.
In order to reduce the non-specific staining possible trouble shooting steps could include : 1) shorten the antibody incubation time, 2) reducing primary and secondary antibody concentrations, and 3) increasing blocking time or try BSA instead of milk as blocking reagent.
Since the customer had contacted us within 120 days of the purchase, a free of charge replacement, credit or refund was offered.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jun 05 2009