Application
Western blot
Sample
Human Cell lysate - whole cell (kidney (HK2))
Loading amount
10 µg
Specification
kidney (HK2)
Gel Running Conditions
Reduced Denaturing (4-12 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/4000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-Tween
Secondary antibody
Name
Non-Abcam antibody was used: HRP goat anti rabbit (Zymed)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 32 kDa Non-specific: 180, 120 kDa
Additional data
Additional Notes
The marker (lane 11) has the following sizes: 200, 140, 100, 80, 60, 50, 40 (weak), 30 (not visibe on this particular gel).
Lane 1 is the untreated samples as shown before ( ignore other lanes which are treated or knocked down)
Lane 1 is the untreated samples as shown before ( ignore other lanes which are treated or knocked down)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Mar 15 2011