Application
Western blot
Sample
Human Cell lysate - whole cell (MDA-MB-468 cell lysate)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
MDA-MB-468 cell lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Dilution
1/10000
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Anti-rabbit IGG HRP 7074 cell signaling
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL with licor c-digit
Exposure
6 minute(s) and 0 second(s)
Bands
Specific: 31 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Apr 09 2018