Anti-NPL4 抗体 (ab68020)
Key features and details
- Mouse polyclonal to NPL4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-NPL4 antibody
NPL4 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to NPL4 -
由来種
Mouse -
特異性
This antibody is reactive against mammalian transfected lysate. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human NPL4. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ・コントロール
- Transfected 293T cell lysate and human spleen tissue lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab68020の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Detects a band of approximately 68, 75 kDa (predicted molecular weight: 68 kDa).
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 68, 75 kDa (predicted molecular weight: 68 kDa). |
ターゲット情報
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機能
The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. -
組織特異性
Expressed at highest levels in brain, heart, skeletal muscle, kidney and fetal liver. -
パスウェイ
Protein degradation; proteasomal ubiquitin-dependent pathway. -
配列類似性
Belongs to the NPL4 family.
Contains 1 RanBP2-type zinc finger. -
ドメイン
Binds ubiquitinated proteins via its RanBP2-type zinc finger. -
細胞内局在
Cytoplasm > cytosol. Endoplasmic reticulum. Nucleus. Associated with the endoplasmic reticulum and nuclear. - Information by UniProt
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参照データベース
- Entrez Gene: 55666 Human
- Omim: 606590 Human
- SwissProt: Q8TAT6 Human
- Unigene: 464333 Human
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別名
- KIAA1499 antibody
- NPL4 antibody
- NPL4, S. cerevisiae, homolog of antibody
see all
画像
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Anti-NPL4 antibody (ab68020) at 1/500 dilution + human spleen tissue lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 68 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted? -
All lanes : Anti-NPL4 antibody (ab68020) at 1/500 dilution
Lane 1 : NPLOC4 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa
データシートおよび資料
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Datasheet download
参考文献 (0)
ab68020 は論文での使用が確認できていません。