Anti-NPHS2 抗体 (ab50339)
Key features and details
- Rabbit polyclonal to NPHS2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-NPHS2 antibody
NPHS2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NPHS2 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat
交差が予測される動物種: Human -
免疫原
Synthetic peptide corresponding to Human NPHS2 aa 367-383 conjugated to keyhole limpet haemocyanin (Cysteine residue). Synthetic peptide conjugated to KLH by an N-terminal Cysteine residue linker.
Sequence:SKPVEPLNPKKKDSPML
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab50339 is affinity-purified using the immunizing peptide immobilized on agarose. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab50339の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (5) |
Use a concentration of 0.2 µg/ml.
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WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 42 kDa. Additional weak bands may be detected when immunoblotting various extract preparations. Detection of podocin (doublet) by immunoblotting is specifically inhibited with the immunizing peptide. The upper band may represent a post-translational modified variant.
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特記事項 |
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IHC-P
Use a concentration of 0.2 µg/ml. |
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 42 kDa. Additional weak bands may be detected when immunoblotting various extract preparations. Detection of podocin (doublet) by immunoblotting is specifically inhibited with the immunizing peptide. The upper band may represent a post-translational modified variant. |
ターゲット情報
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機能
Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton. -
組織特異性
Almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli. -
関連疾患
Defects in NPHS2 are the cause of nephrotic syndrome type 2 (NPHS2) [MIM:600995]. It is a renal disorder characterized clinically by childhood onset of proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. The disorder is resistant to steroid treatment and progresses to end-stage renal failure in the first or second decades. Some patients show later onset of the disorder. -
配列類似性
Belongs to the band 7/mec-2 family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 7827 Human
- Entrez Gene: 170484 Mouse
- Entrez Gene: 170672 Rat
- Omim: 604766 Human
- SwissProt: Q9NP85 Human
- SwissProt: Q91X05 Mouse
- SwissProt: Q8K4G9 Rat
- Unigene: 412710 Human
see all -
別名
- nephrosis 2 antibody
- Nephrosis 2, idiopathic, steroid resistant (podocin) antibody
- nephrosis 2, idiopathic, steroid resistant antibody
see all
画像
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All lanes : Anti-NPHS2 antibody (ab50339) at 1 µg/ml
Lane 1 : Rat glomeruli tissue extract
Lane 2 : Rat glomeruli tissue extract with Immunizing peptide
Secondary
All lanes : Anti_rabbit IgG-peroxidase
Predicted band size: 42 kDa -
Immunohistochemical analysis of formalin-fixed, paraffn-embedded rat kidney tissue using ab50339 at 0.2 μg/ml.
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Immunohistochemical analysis of formalin-fixed, paraffn-embedded mouse kidney tissue using ab50339 at 0.2 μg/ml.
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Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling NPHS2 with ab50339 at 0.2 µg/ml.
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Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling NPHS2 with ab50339 at 0.2 µg/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (83)
ab50339 は 83 報の論文で使用されています。
- Murphy C et al. Comparison of human recombinant protein coatings and fibroblast-ECM to Matrigel for induced pluripotent stem cell culture and renal podocyte differentiation. ALTEX 40:141-159 (2023). PubMed: 35791294
- Lv F et al. CD36 aggravates podocyte injury by activating NLRP3 inflammasome and inhibiting autophagy in lupus nephritis. Cell Death Dis 13:729 (2022). PubMed: 35999224
- Shen Q et al. Integrated genomic, transcriptomic and metabolomic analysis reveals MDH2 mutation-induced metabolic disorder in recurrent focal segmental glomerulosclerosis. Front Immunol 13:962986 (2022). PubMed: 36159820
- Kalejaiye TD et al. SARS-CoV-2 Employ BSG/CD147 and ACE2 Receptors to Directly Infect Human Induced Pluripotent Stem Cell-Derived Kidney Podocytes. Front Cell Dev Biol 10:855340 (2022). PubMed: 35517495
- Mou X et al. A Biomimetic Electrospun Membrane Supports the Differentiation and Maturation of Kidney Epithelium from Human Stem Cells. Bioengineering (Basel) 9:N/A (2022). PubMed: 35621466