Anti-NOX3 抗体 (ab81864)
Key features and details
- Rabbit polyclonal to NOX3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-NOX3 antibody
NOX3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NOX3 -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Dog -
免疫原
Synthetic peptide corresponding to Human NOX3 aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available asab101218) -
ポジティブ・コントロール
- This antibody gave a positive signal in HeLa and HEK293 whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab81864の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 64 kDa).
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ICC/IF |
Use a concentration of 1 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 64 kDa). |
ICC/IF
Use a concentration of 1 µg/ml. |
ターゲット情報
- Information by UniProt
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参照データベース
- Entrez Gene: 50508 Human
- Omim: 607105 Human
- SwissProt: Q9HBY0 Human
- Unigene: 247776 Human
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別名
- EC 1.6.3.- antibody
- GP91 3 antibody
- GP91-3 antibody
see all
画像
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All lanes : Anti-NOX3 antibody (ab81864) at 1 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 64 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?
Additional bands at: 105 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Abcam recommends that this antibody is blocked with milk rather than BSA. -
ICC/IF image of ab81864 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81864, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% MCF7 fixed (10 min) MCF7 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa cells at 1µg/ml
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab81864 は 3 報の論文で使用されています。
- Kim HK et al. Chalcone suppresses tumor growth through NOX4-IRE1a sulfonation-RIDD-miR-23b axis. Redox Biol 40:101853 (2021). PubMed: 33445069
- Liu Z et al. IFI6 depletion inhibits esophageal squamous cell carcinoma progression through reactive oxygen species accumulation via mitochondrial dysfunction and endoplasmic reticulum stress. J Exp Clin Cancer Res 39:144 (2020). PubMed: 32727517
- Plantinga TS et al. Rare NOX3 Variants Confer Susceptibility to Agranulocytosis During Thyrostatic Treatment of Graves' Disease. Clin Pharmacol Ther N/A:N/A (2017). Human . PubMed: 28486791