Anti-NMDAR2B 抗体 (ab73001)
Key features and details
- Rabbit polyclonal to NMDAR2B
- Suitable for: WB, ICC/IF
- Reacts with: Rat
- Isotype: IgG
製品の概要
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製品名
Anti-NMDAR2B antibody
NMDAR2B 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NMDAR2B -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Rat
交差が予測される動物種: Mouse, Chicken, Cow, Dog, Human, Non human primates -
免疫原
Synthetic peptide from the N-terminus of rat NMDAR2B.
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ポジティブ・コントロール
- Rat hippocampal lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.50
Constituents: 0.01% BSA, 50% Glycerol, 0.87% Sodium chloride, 0.238% HEPES -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab73001の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 166 kDa).
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ICC/IF |
1/500.
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特記事項 |
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WB
1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 166 kDa). |
ICC/IF
1/500. |
ターゲット情報
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機能
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. -
組織特異性
Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia. -
配列類似性
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily. -
細胞内局在
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 417965 Chicken
- Entrez Gene: 537804 Cow
- Entrez Gene: 494009 Dog
- Entrez Gene: 2904 Human
- Entrez Gene: 14812 Mouse
- Entrez Gene: 24410 Rat
- Omim: 138252 Human
- SwissProt: Q5R1P3 Dog
see all -
別名
- AW490526 antibody
- EIEE27 antibody
- Glutamate [NMDA] receptor subunit epsilon 2 antibody
see all
画像
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Anti-NMDAR2B antibody (ab73001) at 1/1000 dilution + rat hippocampal lysate at 10 µg
Predicted band size: 166 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Additional bands at: <68 kDa, 120 kDa. We are unsure as to the identity of these extra bands. -
ab73001 diluted 1/500 staining NMDAR2B on 14DIV cultured rat cortical neurons fixed in methanol at - 20oC for 10 minutes. Sections were also labelled with an anti-PSD95 antibody in red.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab73001 は 3 報の論文で使用されています。
- Song Y et al. A novel mechanism of synaptic and cognitive impairments mediated via microRNA-30b in Alzheimer's disease. EBioMedicine N/A:N/A (2018). PubMed: 30522932
- Zhang J & Chen C Alleviation of Neuropathology by Inhibition of Monoacylglycerol Lipase in APP Transgenic Mice Lacking CB2 Receptors. Mol Neurobiol 55:4802-4810 (2018). PubMed: 28733897
- Zhang J et al. Synaptic and cognitive improvements by inhibition of 2-AG metabolism are through upregulation of microRNA-188-3p in a mouse model of Alzheimer's disease. J Neurosci 34:14919-33 (2014). WB ; Mouse . PubMed: 25378159