Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lymph node)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Both Tris-EDTA and Citrate-Sodium
Specification
Lymph node
Blocking step
(agent) for 15 minute(s) · Concentration: 0.25% · Temperature: RT°C
Fixative
Formaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.25% Casein 1xTBS 0.05% Tween
Secondary antibody
Name
Non-Abcam antibody was used: Immunologic BrightVision Poly-HRP-Anti-Mouse
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
No clear staining in neither lymph node, bone marrow nor appendicitis tissues
Abcam response
Thank you for sharing this data with us. ab35033 has not previously been tested in IHC-P and based on this abreview, it looks like this antibody does not work in this application, under these conditions. Abcam would welcome feedback from other customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Aug 12 2015