Anti-NG2 抗体 [132.38] (ab50009)
Key features and details
- Mouse monoclonal [132.38] to NG2
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG1
製品の概要
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製品名
Anti-NG2 antibody [132.38]
NG2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [132.38] to NG2 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Rat
非交差種: Mouse -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Rat brain, spinal cord and hippocampus tissue lysate.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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精製度
Protein G purified -
一次抗体 備考
This antibody is derived from the hybridoma 132.38 produced by the fusion of mouse myeloma cells (P3XAg8.653) and splenocytes from BALB/c mice. -
ポリ/モノ
モノクローナル -
クローン名
132.38 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab50009の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 5 µg/ml. Detects a band of approximately 251 kDa (predicted molecular weight: 251 kDa).
Abcam recommends using BSA as the blocking agent. |
特記事項 |
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WB
Use a concentration of 5 µg/ml. Detects a band of approximately 251 kDa (predicted molecular weight: 251 kDa). Abcam recommends using BSA as the blocking agent. |
ターゲット情報
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機能
Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades. -
組織特異性
Detected only in malignant melanoma cells. -
配列類似性
Contains 15 CSPG (NG2) repeats.
Contains 2 laminin G-like domains. -
翻訳後修飾
O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4.
Phosphorylation by PRKCA regulates its subcellular location and function in cell motility. -
細胞内局在
Apical cell membrane. Cell projection > lamellipodium membrane. Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes. - Information by UniProt
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参照データベース
- Entrez Gene: 81651 Rat
- SwissProt: Q00657 Rat
- Unigene: 10381 Rat
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別名
- 4732461B14Rik antibody
- AN2 antibody
- AN2 proteoglycan antibody
see all
画像
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All lanes : Anti-NG2 antibody [132.38] (ab50009) at 1 µg/ml
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat spinal cord tissue lysate
Lane 3 : Rat hippocampus tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 251 kDa
Observed band size: 270 kDa why is the actual band size different from the predicted?
Additional bands at: 31 kDa (possible non-specific binding), 41 kDa (possible non-specific binding)
Exposure time: 20 minutes
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (48)
ab50009 は 48 報の論文で使用されています。
- Suo L et al. METTL3-mediated N6-methyladenosine modification governs pericyte dysfunction during diabetes-induced retinal vascular complication. Theranostics 12:277-289 (2022). PubMed: 34987645
- Gao X et al. PD-L1 signaling in reactive astrocytes counteracts neuroinflammation and ameliorates neuronal damage after traumatic brain injury. J Neuroinflammation 19:43 (2022). PubMed: 35135580
- Freitas F & Attwell D Pericyte-mediated constriction of renal capillaries evokes no-reflow and kidney injury following ischaemia. Elife 11:N/A (2022). PubMed: 35285797
- Boccazzi M et al. The immune-inflammatory response of oligodendrocytes in a murine model of preterm white matter injury: the role of TLR3 activation. Cell Death Dis 12:166 (2021). PubMed: 33558485
- Kanaan NM & Grabinski T Neuronal and Glial Distribution of Tau Protein in the Adult Rat and Monkey. Front Mol Neurosci 14:607303 (2021). PubMed: 33986642