Application
Immunocytochemistry/ Immunofluorescence
Blocking step
10% Goat Serum and 4% BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Sample
Rat Cell (Isolated Cardiac Fibroblasts)
Specification
Isolated Cardiac Fibroblasts
Permeabilization
Yes - PBS 0.05% Tween 20 - 0.1% Triton X-100
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS 0.05% Tween 20 - 4% BSA
Dilution
1/250
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor Goat anti-Rabbit 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500
Additional data
Additional Notes
Cells were fixed with cold 2% Paraformaldehyde for 30min.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Aaron West
Verified customer
投稿 Jun 12 2013