Application
Western blot
Sample
Chicken Tissue lysate - whole (chick embryo brain E5)
Loading amount
60 µg
Specification
chick embryo brain E5
Gel Running Conditions
Reduced Denaturing (7%)
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Other product details
Dilution
1/400
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 120 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mrs. Elena Astanina
Verified customer
投稿 Apr 08 2008