Application
Western blot
Sample
Mouse Cell lysate - whole cell (el4)
Loading amount
100000 cells
Specification
el4
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Dilution
1/1000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: 5% BSA,0.1% Tween20 in PBS
Secondary antibody
Name
Non-Abcam antibody was used: antimouse
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL plus
Exposure
30 second(s)
Bands
Specific: 120, 140 kDa Non-specific: 200 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jun 12 2012