Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (LS174t)
Specification
LS174t
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Bands
Specific: 70 kDa
Exposure
3 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 10 2014