Anti-NEK7 抗体 (ab95873)
Key features and details
- Rabbit polyclonal to NEK7
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-NEK7 antibody
NEK7 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NEK7 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Orangutan -
免疫原
A region between residue 1 and 50 of human NEK7 (NP_598001.1)
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ポジティブ・コントロール
- HeLa and 293T cell lysates
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab95873 was affinity purified using an epitope specific to NEK7 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab95873の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 35 kDa.
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IP |
Use at 5-15 µg/mg of lysate.
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 35 kDa. |
IP
Use at 5-15 µg/mg of lysate. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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組織特異性
Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine. -
配列類似性
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Contains 1 protein kinase domain. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 781355 Cow
- Entrez Gene: 480008 Dog
- Entrez Gene: 140609 Human
- Entrez Gene: 100521742 Pig
- Omim: 606848 Human
- SwissProt: Q8TDX7 Human
- Unigene: 24119 Human
- Unigene: 723303 Human
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別名
- EC 2.7.11.1 antibody
- NEK7 antibody
- NEK7_HUMAN antibody
see all
画像
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All lanes : Anti-NEK7 antibody (ab95873) at 0.4 µg/ml
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Predicted band size: 35 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling NEK7 with ab95873 at 1/1000 (1µg/ml). Detection: DAB.
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Detection of NEK7 by Western Blot of Immunprecipitate. (1) ab95873 at 0.4µg/ml staining NEK7 which had been immunoprecipitated from HeLa whole cell lysate using ab95873 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane), (2) control IgG. Detection: Chemiluminescence with exposure time of 30 seconds.
All lanes : Anti-NEK7 antibody (ab95873) at 1 µg/ml
Lane 1 : IP: ab95873 at 10µg/mg lysate
Lane 2 : IP: Controle IgG at 10µg/mg lysate
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab95873 は 1 報の論文で使用されています。
- Yan Z et al. Inhibition of NEK7 Suppressed Hepatocellular Carcinoma Progression by Mediating Cancer Cell Pyroptosis. Front Oncol 12:812655 (2022). PubMed: 35223495