Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Kidney)
Specification
Kidney
Fixative
PLP
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EGTA buffer
Permeabilization
Yes - saponin
Blocking step
(agent) for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Other product details
Dilution
1/100
Incubation time
18 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Dako, PO448
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/200
Additional data
Abcam response
Thank you for submitting your Abreview. We are very sorry to hear that the antibody did not perform as expected in this tested species and application. We are in touch with the customer to resolve this problem according to our Abpromise guarantee (https://www.abcam.com/abpromise) and to the customer’s satisfaction.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Feb 08 2013