Anti-NDUFS3 抗体 [17D95] (ab14711)
Key features and details
- Mouse monoclonal [17D95] to NDUFS3
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-NDUFS3 antibody [17D95]
NDUFS3 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [17D95] to NDUFS3 -
由来種
Mouse -
アプリケーション
適用あり: WB, Flow Cytmore details -
種交差性
交差種: Mouse, Rat, Cow, Human -
免疫原
Tissue, cells or virus. This information is considered to be commercially sensitive.
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ポジティブ・コントロール
- Human heart mitochondria. In Flow Cytometry, this antibody gave a positive signal in methanol fixed/Tween permeabilised HepG2 cells.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
モノクローナル -
クローン名
17D95 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab14711の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 30 kDa).
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Flow Cyt |
Use 1µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 30 kDa). |
Flow Cyt
Use 1µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. -
配列類似性
Belongs to the complex I 30 kDa subunit family. -
細胞内局在
Mitochondrion inner membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 287327 Cow
- Entrez Gene: 4722 Human
- Entrez Gene: 68349 Mouse
- Entrez Gene: 295923 Rat
- Omim: 603846 Human
- SwissProt: P23709 Cow
- SwissProt: O75489 Human
- SwissProt: Q9DCT2 Mouse
see all -
別名
- CI 30 antibody
- CI 30KD antibody
- CI-30kD antibody
see all
画像
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All lanes : Anti-NDUFS3 antibody [17D95] (ab14711) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lane 3 : Heart (Mouse) Tissue Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Additional bands at: 57 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
The band observed at 26 kDa could potentially be a cleaved form of NDUFS3 due to the presence of a 36 amino acid transit peptide. -
All lanes : Anti-NDUFS3 antibody [17D95] (ab14711) at 1/1000 dilution
Lanes 1-2 : Whole cell lysates from NDUFS3 KO cells.
Lanes 3-4 : Whole cell lysates from NDUFS3 WT cells.
Lanes 5-6 : Isolated mitochondria from NDUFS3 KO cells.
Lanes 7-8 : Isolated mitochondria from NDUFS3 WT cells.
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-mouse polyclonal HRP conjugate at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Additional bands at: 22 kDa (possible non-specific binding), 40 kDa (possible non-specific binding)
Exposure time: 1 minute
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Overlay histogram showing HepG2 cells stained with ab14711 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14711, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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All lanes : Anti-NDUFS3 antibody [17D95] (ab14711)
Lane 1 : Isolated mitochondria from Human heart at 5 µg
Lane 2 : Isolated mitochondria from Bovine heart at 4 µg
Lane 3 : Isolated mitochondria from Rat heart at 10 µg
Lane 4 : Isolated mitochondria from Mouse heart at 10 µg
Secondary
All lanes : Goat anti-Mouse IgG
Predicted band size: 30 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (98)
ab14711 は 98 報の論文で使用されています。
- Murari A et al. IDH2-mediated regulation of the biogenesis of the oxidative phosphorylation system. Sci Adv 8:eabl8716 (2022). PubMed: 35544578
- Benegiamo G et al. COX7A2L genetic variants determine cardiorespiratory fitness in mice and human. Nat Metab 4:1336-1351 (2022). PubMed: 36253618
- Hossain KFB et al. The membrane domain of respiratory complex I accumulates during muscle aging in Drosophila melanogaster. Sci Rep 12:22433 (2022). PubMed: 36575244
- Gumeni S et al. Nrf2 activation induces mitophagy and reverses Parkin/Pink1 knock down-mediated neuronal and muscle degeneration phenotypes. Cell Death Dis 12:671 (2021). PubMed: 34218254
- Sladowska M et al. Proteasome activity contributes to pro-survival response upon mild mitochondrial stress in Caenorhabditis elegans. PLoS Biol 19:e3001302 (2021). PubMed: 34252079