Anti-NDUFA1 抗体 (ab131423)
Key features and details
- Rabbit polyclonal to NDUFA1
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-NDUFA1 antibody
NDUFA1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NDUFA1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Rat, Human -
免疫原
Synthetic peptide corresponding to a sequence at the C terminal of Human NDUFA1, different from the related rat and mouse sequences by one amino acid.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.1% Dibasic monohydrogen sodium phosphate, 0.45% Sodium chloride -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab131423の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 8 kDa.
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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特記事項 |
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WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 8 kDa. |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. -
組織特異性
Primarily expressed in heart and skeletal muscle. -
関連疾患
Defects in NDUFA1 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. -
配列類似性
Belongs to the complex I NDUFA1 subunit family. -
細胞内局在
Mitochondrion inner membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 4694 Human
- Entrez Gene: 363441 Rat
- Omim: 300078 Human
- SwissProt: O15239 Human
- Unigene: 534168 Human
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別名
- CI MWFE antibody
- CI-MWFE antibody
- Complex I MWFE antibody
see all
画像
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All lanes : Anti-NDUFA1 antibody (ab131423) at 0.5 µg/ml
Lane 1 : Rat Heart tissue lysate
Lane 2 : Rat Brain tissue lysate
Lane 3 : Rat Liver tissue lysate
Lane 4 : Rat Kidney tissue lysate
Lane 5 : HT1080 Cell Lysate
Predicted band size: 8 kDa -
Immunohistochemical analysis of paraffin-embedded Rat Cardiac Muscle Tissue labelling NDUFA1 with ab131423 at 1µg/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab131423 は 2 報の論文で使用されています。
- Mulcahy MJ et al. Brain Region-Specific nAChR and Associated Protein Abundance Alterations Following Chronic Nicotine and/or Menthol Exposure. J Proteome Res 19:36-48 (2020). PubMed: 31657575
- Wu S et al. The circ_0004463/miR-380-3p/FOXO1 axis modulates mitochondrial respiration and bladder cancer cell apoptosis. Cell Cycle 19:3563-3580 (2020). PubMed: 33283616