Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - 0.01%TritonX-100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution
1/500
Additional data
Additional Notes
Trans-cardial perfusions were performed using 4% PFA (in 1X PBS) followed by 4 hour post-fix in same solution. Tissue was then cryoprotected in a 20% sucrose section and a cryostat was used to cut coronal sections at 70 um. Strong labeling is evident surrounding neuronal cell bodies.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ben Siemsen
Verified customer
投稿 Jun 22 2015