Anti-NCF1/p47-phox (phospho S359) 抗体 (ab74095)
Key features and details
- Rabbit polyclonal to NCF1/p47-phox (phospho S359)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-NCF1/p47-phox (phospho S359) antibody
NCF1/p47-phox 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NCF1/p47-phox (phospho S359) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human NCF1/p47-phox (phospho S359).
Sequence:Q-R-Sp-K-P
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ポジティブ・コントロール
- Human colon carcinoma tissue, HeLa cells and HeLa cell extracts treated with nocodazole (1ug/ml, 18hours) or TSA (400nM, 24hours).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab74095 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab74095の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
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IHC-P |
1/50 - 1/100.
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ICC/IF |
1/500 - 1/1000.
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). |
IHC-P
1/50 - 1/100. |
ICC/IF
1/500 - 1/1000. |
ターゲット情報
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機能
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). -
関連疾患
Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections. -
配列類似性
Contains 1 PX (phox homology) domain.
Contains 2 SH3 domains. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 653361 Human
- Omim: 608512 Human
- SwissProt: P14598 Human
- Unigene: 647047 Human
- Unigene: 655201 Human
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別名
- 47 kDa autosomal chronic granulomatous disease protein antibody
- 47 kDa neutrophil oxidase factor antibody
- NADPH oxidase organizer 2 antibody
see all
画像
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All lanes : Anti-NCF1/p47-phox (phospho S359) antibody (ab74095) at 1/500 dilution
Lane 1 : HeLa cell extracts treated
with nocodazole (1ug/ml, 18hours)
Lane 2 : HeLa cell extracts treated
with TSA (400nM, 24hours)
Lane 3 : HeLa cell extracts treated
with nocodazole (1ug/ml, 18hours) and with TSA (400nM, 24hours) with the immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 45 kDa
Observed band size: 45 kDa -
ab74095 at 1/50 dilution staining NCF1/p47-phox in human colon carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
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ab74095 at 1/500 dilution staining NCF1/p47-phox in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab74095 は 2 報の論文で使用されています。
- Cao X et al. Effects of Probiotics BaSC06 on Intestinal Digestion and Absorption, Antioxidant Capacity, Microbiota Composition, and Macrophage Polarization in Pigs for Fattening. Front Vet Sci 7:570593 (2020). PubMed: 33240950
- Fischer MT et al. NADPH oxidase expression in active multiple sclerosis lesions in relation to oxidative tissue damage and mitochondrial injury. Brain 135:886-99 (2012). IHC-P ; Human . PubMed: 22366799