Application
Western blot
Sample
Chinese hamster Cell lysate - nuclear (Cell line CHO)
Gel Running Conditions
Non-reduced Denaturing (gel 4-12% Bis Acrylamide)
Loading amount
20 µg
Specification
Cell line CHO
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Donkey anti rabbit HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Clarity western ECL substrate
Exposure
20 second(s)
Bands
Specific: 155 kDa
Positive control
We used glial cell line for positive control
Additional data
Additional Notes
This antibody don't work in CHO cell line
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Apr 27 2017