Anti-NCAM1 抗体 (ab118291)
Key features and details
- Goat polyclonal to NCAM1
- Suitable for: Flow Cyt, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-NCAM1 antibody
NCAM1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to NCAM1 -
由来種
Goat -
特異性
ab118291 is expected to recognize reported isoform 2 (NP_851996.2) only. -
アプリケーション
適用あり: Flow Cyt, WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Cow, Pig -
免疫原
Synthetic peptide:
KASWTRPEKQETLD
with a Cysteine residue linker, corresponding to internal sequence amino acids 353-366 of Human NCAM (NP_851996.2). -
ポジティブ・コントロール
- WB: Human brain frontal cortex lysates. IHC: human adrenal gland tissue. Flow Cyt: U251 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab118291 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab118291の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
Use a concentration of 10 µg/ml.
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WB |
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 170 kDa (predicted molecular weight: 94 kDa).
A 1 hour primary incubation is recommended for this product. The observed molecular weight corresponds to the glycosylated form. |
|
IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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Flow Cyt
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 170 kDa (predicted molecular weight: 94 kDa). A 1 hour primary incubation is recommended for this product. The observed molecular weight corresponds to the glycosylated form. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. -
配列類似性
Contains 2 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 281941 Cow
- Entrez Gene: 4684 Human
- Entrez Gene: 24586 Rat
- Omim: 116930 Human
- SwissProt: P31836 Cow
- SwissProt: P13591 Human
- SwissProt: P13596 Rat
- Unigene: 503878 Human
see all -
別名
- antigen MSK39 identified by monoclonal antibody 5.1H11 antibody
- antigen recognized by monoclonal antibody 5.1H11 antibody
- CD56 antibody
see all
画像
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Anti-NCAM1 antibody (ab118291) at 0.01 µg/ml + Human frontal cortex lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 94 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
The observed molecular weight corresponds to the glycosylated form of the protein. -
Flow cytometric analysis of paraformaldehyde fixed U251 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/ml) followed by Alexa Fluor® 488 secondary antibody (1 µg/ml).
IgG control: Non-immunized goat IgG (black line) followed by Alexa Fluor 488® secondary antibody.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab118291 は論文での使用が確認できていません。