Application
Western blot
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/500
Incubation time
4 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: 2.5% milk in PBS-Tween20
Secondary antibody
Name
Non-Abcam antibody was used: Alexa 647-conjugated goat anti-mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Dilution
1/1000
Detection
Detection method
fluorescence scanning
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 140, 160 kDa Non-specific: 35, 40, 45 kDa
Additional data
Additional Notes
The secondary antibody (Alexa647-conjugated) was detected using a fluorescence scanner equipped with a red laser (633 nm), at a PMT of 700 V.
The antibody detects specific NCAM bands, however it also binds unspecific lower molecular weight proteins.
The antibody detects specific NCAM bands, however it also binds unspecific lower molecular weight proteins.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Dec 03 2012