Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Testis, embryonic day 15.5)
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Specification
Testis, embryonic day 15.5
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C
Dilution
1/500
Secondary antibody
Dilution
1/500
Name
Abcam antibody:Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed
Additional data
Additional Notes
We were unable to detect a clear signal above backround level. Phalloidin (red), which stains F-actin was used as a counterstain.
Abcam response
Thank you for sharing this data with us. A previous batch of ab70000 was successfully used in IHC-P on testis tissue sections by Kusz KM et al., 2009. However, when tested on human brain tissue sections ab70000 gave negative results in IHC-P (data not shown). Based on this abreview, we would suggest that ab70000 is not suitable for IHC, under these experimental condition.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Bryan Niedenberger
Verified customer
投稿 Apr 19 2016