Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (12% page)
Sample
Human Cell lysate - whole cell (epithelioid pancreatic carcinoma - panc-1 atcc cel)
Specification
epithelioid pancreatic carcinoma - panc-1 atcc cel
Treatment
mock transfected - empty vector
Blocking step
Milk as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: skim milk 5%
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: anti rabbit HRP , Jackson Labs
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/30000
Detection
Detection method
supersignal west pico, PIERCE
Bands
Specific: ~80 kDa
Exposure
10 minute(s) and 0 second(s)
Positive control
hela cells whole lysate. The gel running pattern had marginal downward tendency (2 irrelevant lanes were cut from the figure, but the size is the same as the two closer to the ladder)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 17 2014