Anti-N4BP1 抗体 (ab106649)
Key features and details
- Rabbit polyclonal to N4BP1
- Suitable for: ICC, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-N4BP1 antibody
N4BP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to N4BP1 -
由来種
Rabbit -
特異性
At least three isoforms of N4BP1 are known to exist; ab106649 will only detect the longest isoform. -
アプリケーション
適用あり: ICC, WBmore details -
種交差性
交差種: Human -
免疫原
An 18 amino acid synthetic peptide near the N terminus of Human N4BP1 (NP_694574).
-
ポジティブ・コントロール
- WB: Jurkat and K562 whole cell lysates; ICC: HeLa cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at 4°C (stable for up to 12 months). -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab106649の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC |
Use a concentration of 10 - 20 µg/ml.
|
|
WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 100 kDa.
|
特記事項 |
---|
ICC
Use a concentration of 10 - 20 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 100 kDa. |
ターゲット情報
-
機能
Inhibitor of the E3 ubiquitin-protein ligase ITCH. Acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates. -
組織特異性
Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary. -
配列類似性
Belongs to the N4BP1 family. -
翻訳後修飾
Monoubiquitinated by NEDD4. Polyubiquitinated, leading to its degradation by the proteasome. Sumoylated by SUMO1, abrogating polyubiquitination and subsequent degradation. Desumoylated by SENP1, leading to accumulation in PML nuclear bodies.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus > nucleolus. Nucleus > PML body. Primarily localizes to the nucleolus. Also localizes to the PML nuclear bodies, when desumoylated. - Information by UniProt
-
参照データベース
- Entrez Gene: 9683 Human
- SwissProt: O75113 Human
- Unigene: 511839 Human
-
別名
- FLJ31821 antibody
- KIAA0615 antibody
- MGC176730 antibody
see all
画像
-
All lanes : Anti-N4BP1 antibody (ab106649) at 1 µg/ml
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 100 kDa
Exposure time: 30 secondsPrimary incubation for 1 hour at room temperature in 5% NFDM/TBST.
-
Immunofluorescence of N4BP1 in HeLa cells with ab106649 at 20 ug/mL.
-
Immunocytochemistry of N4BP1 in HeLa cells with ab106649 at 10 ug/mL.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab106649 は論文での使用が確認できていません。