Application
Western blot
Sample
Human Cell lysate - whole cell (MCF-7 cell)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
30 µg
Specification
MCF-7 cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5%BSA
Dilution
1/20000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: HRP-Goat Anti-Rabbit IgG(H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
15 second(s)
Bands
Specific: 100 kDa
Additional data
Additional Notes
1-4. 4 siRNAs were designed to knockdown N-cadherin. 5. siRNA NC was added which shoul not effect level of N-cadherin.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Mar 11 2016