Anti-Myosin Phosphatase (phospho T696) 抗体 (ab59202)
Key features and details
- Rabbit polyclonal to Myosin Phosphatase (phospho T696)
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Myosin Phosphatase (phospho T696) antibody
Myosin Phosphatase 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Myosin Phosphatase (phospho T696) -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human Myosin Phosphatase aa 650-750 (phospho T696).
Database link: O14974 -
ポジティブ・コントロール
- Human breast carcinoma tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab59202の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration.
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特記事項 |
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IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Regulates myosin phosphatase activity. -
組織特異性
Expressed in striated muscles, specifically in type 2a fibers (at protein level). -
配列類似性
Contains 6 ANK repeats. -
発生段階
Induced by 2-fold during pregnancy, including in abdominus rectus muscle. -
ドメイン
Heterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase. -
翻訳後修飾
Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1. -
細胞内局在
Cytoplasm. Along actomyosin filaments and stress fibers. - Information by UniProt
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参照データベース
- Entrez Gene: 4659 Human
- Entrez Gene: 17931 Mouse
- Entrez Gene: 116670 Rat
- Omim: 602021 Human
- SwissProt: O14974 Human
- SwissProt: Q9DBR7 Mouse
- SwissProt: Q10728 Rat
- Unigene: 49582 Human
see all -
別名
- M130 antibody
- MBS antibody
- Myosin binding subunit antibody
see all
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab59202 は 2 報の論文で使用されています。
- Yin Y et al. Expression of MYPT1, CPI-17 and MLC20 in ileum of neonatal mouse NEC model and its significance. Exp Ther Med 14:2221-2227 (2017). PubMed: 28962146
- Sastre E et al. Decompensated liver cirrhosis and neural regulation of mesenteric vascular tone in rats: role of sympathetic, nitrergic and sensory innervations. Sci Rep 6:31076 (2016). WB ; Rat . PubMed: 27484028