Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (hESC-derived cardiomyocyte)
Specification
hESC-derived cardiomyocyte
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Other product details
Dilution
1/250
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 4% goat serum in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti Mouse CY3
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/250
Additional data
Additional Notes
Antibody is not specific for MYL2 (MLC2v) as it is expressed in non-cardiomyocytes (fibroblasts) and does not show sarcomeric patterning.
Abcam response
Thank you for submitting this abreview. We have not previously tested this myosin light chain 2 antibody in IF and would welcome feedback from other customers using ab89594 in this application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Dec 03 2010