製品の概要

  • 製品名
    Anti-Myoferlin antibody [7D2]
    Myoferlin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [7D2] to Myoferlin
  • アプリケーション
    適用あり: ICC/IF, IHC-P, Flow Cyt, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide corresponding to Human Myoferlin (N terminal).
    Database link: Q9NZM1

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Constituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    7D2
  • アイソタイプ
    IgG2a
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab76746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/100 - 1/1000.
Flow Cyt 1/100. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
WB 1/500 - 1/5000. Predicted molecular weight: 235 kDa.

ターゲット情報

  • 機能
    Calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. Involved in endocytic recycling. Implicated in VEGF signal transduction by regulating the levels of the receptor KDR.
  • 組織特異性
    Expressed in myoblast and endothelial cells (at protein level). Highly expressed in cardiac and skeletal muscles. Also present in lung, and at very low levels in kidney, placenta and brain.
  • 配列類似性
    Belongs to the ferlin family.
    Contains 5 C2 domains.
  • ドメイン
    The C2 domain 1 associates with lipid membranes in a calcium-dependent manner.
  • 細胞内局在
    Cell membrane. Nucleus membrane. Cytoplasmic vesicle membrane. Concentrated at the membrane sites of both myoblast-myoblast and myoblast-myotube fusions. Detected at the plasmalemma in endothelial cells lining intact blood vessels (By similarity). Found at nuclear and plasma membranes. Enriched in undifferentiated myoblasts near the plasma membrane in puncate structures.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Fer 1 like 3, myoferlin (C. elegans) antibody
    • Fer 1 like family member 3 antibody
    • Fer 1 like protein 3 antibody
    • Fer-1-like protein 3 antibody
    • FER1L3 antibody
    • myoF antibody
    • MYOF_HUMAN antibody
    • Myoferlin antibody
    see all

画像

  • ICC/IF image of ab76746 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76746, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HeLa cells stained with ab76746 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76746, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Immunohistochemical analysis of Human airway tissue, staining Myoferlin with ab76746 at 1/500 dilution. Non-specific binding was blocked with 10% horse serum and slides were incubated overnight with primary antibody. Staining was detected with DAB.

参考文献

This product has been referenced in:
  • Song DH  et al. Myoferlin expression in non-small cell lung cancer: Prognostic role and correlation with VEGFR-2 expression. Oncol Lett 11:998-1006 (2016). IHC . Read more (PubMed: 26893682) »
  • Lund TC  et al. sdf1 Expression reveals a source of perivascular-derived mesenchymal stem cells in zebrafish. Stem Cells 32:2767-79 (2014). Flow Cyt ; Zebrafish . Read more (PubMed: 24905975) »

See all 3 Publications for this product

レビューと Q&A

Application
Flow Cytometry
Sample
Mouse Cell (B16 melanoma tumour cell line)
Permeabilization
No
Gating Strategy
live cells, singlets
Specification
B16 melanoma tumour cell line
Preparation
Cell harvesting/tissue preparation method: cells were trypsinised, washed with PBS
Sample buffer: PBS
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投稿 Sep 12 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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