Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (muscle, satellite cell)
Permeabilization
No
Specification
muscle, satellite cell
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
Formaldehyde
Other product details
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% rabbit serum, DPBS
Dilution
1/200
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Rabbit anti-Mouse IgG (H+L) Secondary Antibody
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Abcam response
Many thanks for your valuable feedback. Abcam has not validated this antibody in Immunocytochemistry/ Immunofluorescence with pig samples as used in this Abreview. It appears that under these experimental conditions, ab16148 does not specifically detect MyoD1 in chicken. We would welcome further feedback of other researchers. For more information, please contact our scientific support team.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 28 2016