Application
ChIP
Sample
Mouse Cell lysate - whole cell (C2C12 cell line)
Negative control
Rabbit IgG
Specification
C2C12 cell line
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 11 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde (final concent
Duration of cross-linking step: 11 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde (final concent
Positive control
Bmal1 Ab and A MyoD1 Ab from other company
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C
Additional data
Additional Notes
This MyoD1 Ab is not good for ChIP assay. Two ChIP diifferent protocols have been used. One is stringent and the other is less stringent. In both the stringent and less stringent conditions, ct numbers for this MyoD1 Ab is same as IgG at Tef promoter region and Dbp promoter regions.
Abcam response
This antibody was not previously tested for ChIP and therefore may not be effective in this application. Further optimization may improve the results.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Aug 06 2014