Application
Western blot
Sample
Mouse Tissue lysate - other (Brown adipose tissue)
Gel Running Conditions
Non-reduced Denaturing (4-12% Bis-Tris MOPS buffer, extended run (60 mins at 180v))
Loading amount
25 µg
Specification
Brown adipose tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 22°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 4% BSA TBS-T
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: LiCOR
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Detection
Detection method
LiCOR
Negative control
BAT soluble fraction (cytosol)
Exposure
18 minute(s) and 0 second(s)
Bands
Specific: 25-28 kDa Non-specific: None kDa
Positive control
BAT insoluble fraction (pellet)
Additional data
Additional Notes
Cytosolic and insoluble pellet. Insoluble fraction was boiled in SDS to solubilise nuclear and membrane bound proteins.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Alice Pollard
Verified customer
投稿 May 09 2017