Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Blood smear)
Permeabilization
No
Specification
Blood smear
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1%
Fixative
Formaldehyde
Other product details
Incubation time
2 hour(s) and 0 minute(s)
Dilution
1/100
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Goat anti-rabbit AF488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Human blood smear dried overnight and fixed in 3.5% formaldehyde in PBS.
Polymorphonuclear cell (white arrow) has positivity while mononuclears do not.
Scale bar: 50um
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Simon Cleary
Verified customer
投稿 Dec 01 2015